Supplemental Figure 4 Effects of TRIM3 upregulating and downregulation in mammalian models. from <i>Drosophila</i> Brat and Human Ortholog TRIM3 Maintain Stem Cell Equilibrium and Suppress Brain Tumorigenesis by Attenuating Notch Nuclear Transport

(A) Western blot of N08-74 VC and N08-74-TRIM3 expressing cells and 13113 VC and 13113-TRIM3 expressing cells. TRIM3 expression does not increase the glial cell marker GFAP or neuronal marker TUJ-1. (B) TRIM3 sh-RNA in NHNP cells reduces the TRIM3 expression. TRIM3 suppression does not increase total or cytosolic NICD but increases NICD nuclear localization (B,C,D). Nuclear and cytosolic extracts show presence of TRIM3 in the cytosol, but not in nucleus (E).