Supplementary Figures S1-S7 from Tissue Factor Induced by Epithelial–Mesenchymal Transition Triggers a Procoagulant State That Drives Metastasis of Circulating Tumor Cells

Supplementary Figure S1: Optimization of the triple immunostaining on different cell lines with known status of cytokeratins, TF and vimentin expression. Supplementary Figure S2: TF expression and pro-coagulant activity of EMT inducible cell lines. Supplementary Figure S3: Impact of TF blocking antibody on coagulant activity. Supplementary Figure S4: Regulation of TF by EMT transcription factors. Supplementary Figure S5: Induction of TF after de novo expression of Snail in MDA-MB-468. Supplementary Figure S6: Validation of TF shRNA. Supplementary Figure S7: Presence of fibrin fibers around lung colonizing MDA-MB-231 cells as revealed by transmission electron microscopy.