Figure S7 from <i>LINC00261</i> Is an Epigenetically Regulated Tumor Suppressor Essential for Activation of the DNA Damage Response

Figure S7. TCGA LUAD methylation at the FOXA2 locus. A. Individual CpGs are denoted by their Illumina 450K designation. Scale = 0 (no methylation) to 1 (complete methylation). Hg19-based coordinates for each probe are listed below the cg ascension number. Distance from FOXA2 transcript variant 1 Transcription Start Site (TSS) is also shown. "+" is downstream of TSS (within the gene), "-" is upstream of TSS. B. Luciferase assay for activity of FOXA2 promoter in the presence or absence of in vitro SssI methylation. Data is normalized to CpGLess empty vector values from appropriate treatment group. A paired t-test was performed on N=3 biological replicates, each performed in technical triplicate.