Supplementary Figures from Sphingosine Kinase 1 Signaling Promotes Metastasis of Triple-Negative Breast Cancer

SPHK1 expression in TNBC tumors and cell-lines. SPHK1 overexpression increase metastatic related properties. SPHK1 knockdown has no significant difference in cell proliferation but decrease invasion and migration potential in vitro. SPHK1 knockdown has no significant difference in tumor cell proliferation but increase apoptosis. Correlation between SPHK1 and FSCN1 expression. Ectopic expression of FSCN1 rescue the invasive and migrative potential of SPHK1 knockdown cells in vitro. FSCN1 knockdown decreased metastasis related properties in vitro. SPHK1 plays role in increasing the transcriptional rate of the FSCN1 gene transcription but not the mRNA stability. Safingol and Bortezomib treatment on TNBC cells in vitro and in vivo. Inhibition of lung metastasis by safingol plus bortezomib combination treatment when primary tumors were removed at similar sizes.