Supplementary data from Pharmacological Inhibition of Centrosome Clustering by Slingshot-Mediated Cofilin Activation and Actin Cortex Destabilization

Extended experimental procedures Supplementary Fig. S1. Effect of CP-673451 and Crenolanib on centrosome amplification in U2OS cells. Supplementary Fig. S2. Crenolanib prolongs of mitotic duration. Supplementary Fig. S3. CP-673451 activates cofilin in a dose-dependent manner in a variety of cancer cell lines. Supplementary Fig. S4. Inhibition of cofilin phosphorylation by LIMKi3 and Damnacanthal. Supplementary Fig. S5. Immunoblot analysis showing transient overexpression of different cofilin constructs and representative images of transfection efficiencies in U2OS-EGFP-PLK4 cells. Supplementary Fig. S6. Validation of SSH knock-down by real-time PCR. Supplementary Fig. S7. Effect of CP-673451 on SSH1 phosphatase activity. Supplementary Fig. S8. Immunoblot analysis of different cell lines showing that CP-673451 increases Akt and MEK1/2 phosphorylation under normal cultivation conditions. Supplementary Table S2. Average percentages of multipolar telophases in various cell lines after 24 hours of exposure to CP-673451 or crenolanib.