Supplemental Figure 2 Brain tumor in adult Drosophila reduces lifespan and causes motor defect. from <i>Drosophila</i> Brat and Human Ortholog TRIM3 Maintain Stem Cell Equilibrium and Suppress Brain Tumorigenesis by Attenuating Notch Nuclear Transport

(A) Kaplan-Meyer survival analysis of adult female Drosophila shows that brat-RNAi leads to a reduction in mean life expectancy as compared to controls. (B) Adult flies with brain tumor also have reduced negative geotaxis, a test of motor function. (C,D,E,F) PH3 staining in whole brain shows the adult brat-RNAi brain with actively dividing cells, whereas normal brain does not contain dividing cells. N = 20. P<0.0001. (G, H) Low magnification image of control and brat-RNAi brain. Repo-GFP shows significantly higher number tumor-like cells (marked with Tub-cherry) expressing the glial marker Repo in brat-RNAi brain. (I,J) Higher magnification image of brat-RNAi brain showing cells that do not express Tub-cherry, but that show Miranda expression. bratIR is brat-RNAi. Scale bar: 150 um for (C-F), 100 um for (G,H), 10um for (I,J).