Supplementary Figures 1 - 9 from Hedgehog Signaling Regulates Bladder Cancer Growth and Tumorigenicity

PDF file, 596K, Supplemental Figure S1. Validation of the shRNA knockdown efficiencies. Supplemental Figure S2. UC cell lines require HH signaling for proliferation. Supplemental Figure S3. SMO inhibition induces apoptosis in a subset of UC cell lines. Supplemental Figure S4. A 2507-gene signature predicts the HH-dependence of UC cells for proliferation. Supplemental Figure S5. Constitutive HH signaling is required for the anchorage-independent growth of UC cells. Supplemental Figure S6. UC cells maintain HH signaling activity through ligand expression. Supplemental Figure S7. SMO localizes to primary cilia in UC cell lines. Supplemental Figure S8. In situ hybridization (ISH) confirms the positive staining of SHH and GLI1 IHC in human UC. Supplemental Figure S9. Increased expression of SHH and GLI1 in bladders associated with cystitis cystica.