Figure S5. RASSF1A depletion reduces focal adhesion proteins and adhesion capacities in vitro. from RASSF1A Suppresses the Invasion and Metastatic Potential of Human Non–Small Cell Lung Cancer Cells by Inhibiting YAP Activation through the GEF-H1/RhoB Pathway

The HBEC-3 cells were transiently transfected with siNeg, si RASSF1A or siRASSF1AC. The experiences were performed 48 hours after transfection. A. Expression of focal adhesion complex proteins was examined by RT-PCR analysis. B & C. Adhesion strength of the HBEC-3 cells on fibronectin (B) and laminin (C). For all of the histograms, error bars indicate the standard error of the mean (SEM) of at least three independent experiments. *p<0.05, **p<0.01 and ***p<0.001, using an ANNOVA test followed by Dunnett's test.