Supplementary Figure 2 from Cancer-Associated Adipose Tissue Promotes Breast Cancer Progression by Paracrine Oncostatin M and Jak/STAT3 Signaling

Upper panels: Western blot analysis of E-cadherin, beta-catenin, vimentin, fibronectin, pancytokeratin and estrogen receptor alpha (ERα). Left panel: MCF-7 cells treated for 48h with control medium or CM^CAAT. Right panel: MCF-7 cells stably transfected with GFP or OSM cDNA, or treated for 48h with rhOSM (1ng/ml). Tubulin serves as an internal control. Lower left panel: Confocal images of the F-actin cytoskeleton (red), nuclear staining DAPI (blue) and merged signals in MCF-7 cells treated for 48h with control medium or CM^CAAT (scale bar is 20 μm) and confocal images of E-cadherin (green), beta-catenin (red) and merge signals with DAPI in MCF-7 cells treated for 48h with control medium or CM^CAAT (scale bar is 10 μm). Lower right panel: Immunohistochemical staining of MCF-7-GFP and MCF-7-OSM tumor xenografts for E-cadherin, vimentin, pancytokeratin and ERα (scale bar is 25 µm).