Supplementary Figure 5 from Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2

PDF file - 135K, A375 melanoma cells were exposed for 6 hours or 24 Hours to ODQ (3 uM, 10 uM), or the vehicle control dimethylsulfoxide (DMSO). mTOR pathway activation was analyzed by 4EBP1 and p-S6RP western blot. Signals were quantified by densitometry, and the ratio of activated p-4EBP1 and p-S6RP was calculated, normalized against total protein (lower panel). ODQ even at extended time point (24 hours) and high dose (10 uM) had little effect on mTOR pathway activation.