Figure S1 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7

Figure S1. Characterization of P2X7R shRNA B16 cells. A, densitometry of P2X7R protein expression. B, representative Western blot. C, Fura-2 measurement of Benzoyl ATP (BzATP)-triggered intracellular Ca2+ increase. D, Averages intracellular Ca2+ increases in wt and P2X7R-silenced B16 cells. Data are averages + SEM, n = 5. ***, p < 0.001. E, rate of proliferation of wt and P2X7R-silenced B16 cell cultures. Intracellular Ca2+ changes and proliferation rates were measured as described in Adinolfi et al., 2005, Mol Biol Cell 16:3260-3272.