Supplementary Methods from A Wnt-Induced Phenotypic Switch in Cancer-Associated Fibroblasts Inhibits EMT in Colorectal Cancer

In this file we provide additional methods for the described procedures including organoid transduction/transgenesis, biological image processing and image quantification. We provide in-depth information on the RNA sequencing protocol used and describe details of subsequent bioinformatic workflows including 'NicheNet' analysis. Also we provide details on the in silico analysis of CRC patient data and Kaplan-Meier analysis.