Figure S2: Depletion of RASSF1A alone or with RASSF1C results in disappearance of tight junctions and decrease of adherens junction, replaced by diffuse adherent junctions. from RASSF1A Suppresses the Invasion and Metastatic Potential of Human Non–Small Cell Lung Cancer Cells by Inhibiting YAP Activation through the GEF-H1/RhoB Pathway

Three independent air-liquid cultures, each requiring 21 days of growth before fixation and observation, were analyzed. For each culture, 2 slides through the porous membrane and the cells have been performed, those slides being processed at the center of the membrane to avoid artifacts linked to a peripheral location. For siNeg condition (only one stratum of cells) 50 to 70 cells were observed, whereas for siRASSF1A conditions, 50 to 250 cells (several strata of cells) were analyzed. For all of the histograms, error bars indicate the standard error of the mean (SEM) of at least three independent experiments. *p<0.05, **p<0.01, using an ANOVA test followed by Dunnett's test.