Supplementary Figure 2 from MYC Regulation of CHK1 and CHK2 Promotes Radioresistance in a Stem Cell-like Population of Nasopharyngeal Carcinoma Cells

PDF file - 2674K, A, Western blot analysis of c-MYC, CHK1, and CHK2 in vector control and c-MYC+shCHK1/shCHK2 infected PKH26- NPC CNE2 cells. B. EdU proliferation assay of vector control, c-MYC, and c-MYC+shCHK1/CHK2 infected irradiated (8 Gy) PKH26- NPC CNE2 cells. Quantification of the percentages of cells with comet tails following irradiation at different doses. (Data represent the mean�standard deviation (SD); N = 3; *P=0.000; **P=0.064, with a 20 msec exposure time for image acquisition). C. Alkaline comet assay of vector control, c-MYC, and c-MYC+shCHK1/CHK2 infected irradiated (8 Gy) PKH26+NPC CNE2 cells. Quantification of the percentages of cells with comet tails following irradiation at different doses (Data represent the mean�standard deviation (SD); N = 3; *P=0.000; **P=0.102)