Compiled supplemental data from Radiotherapy and CD40 Activation Separately Augment Immunity to Checkpoint Blockade in Cancer

Supplemental Methods: Mouse strains and flow cytometry antibodies Supplemental Figure 1: Individual tumor kinetics of the unirradiated tumor in the PDA model. Tumor kinetics (mean and individual) of the irradiated tumor and survival in the B16-F10 melanoma model. Supplemental Figure 2: Individual tumor kinetics of the unirradiated tumor in antibody depleted mice (Cd4, CD8 and Ly6c) and knockout mice (Morrbid, Batf3, IFN gamma, CD40.) Mean tumor kinetics of the unirradiated tumor and survival in uMT and B220 knockout mice. Supplemental Figure 3: Mean tumor kinetics of the unirradiated tumor and survival in antibody depleted mice (IFNAR) and knockout mice (MyD88, TLR4, IFNAR, Sting.) Supplemental Figure 4: Flow cytometry plots for the top RF variables for the effect of RT Supplemental Figure 5: As for Supplemental figure 4, but for CD40 Supplemental Figure 6: As for supplemental Figure 4, but for the peak response Supplemental Figure 7: Heat map of the RF variables that predict response in irradiated and unirradiated tumors at 24 and 72 hours. Supplemental Figure 8: Histological samples of CD8 T cell infiltrate and SMA expression. Supplemental Figure 9: Graphical representation