Supplementary Information Figure S1 to S5 from HER2-Driven Breast Tumorigenesis Relies upon Interactions of the Estrogen Receptor with Coactivator MED1

Supplementary Information - This file contains Supplemental Procedures, Supplemental References, Supplemental Table1, Supplementary Figure Legends, and Supplementary Figures. Supplemental Procedure describes the methods and PCR primers used in this study. Supplemental References lists the references cited in the supplemental procedures. Supplemental Table1 shows the patients demographics and tumor data. Supplementary Figure Legends describe the legend for each supplementary figure. Figure S1 shows MED1 LxxLL motif mutations reduce cell proliferation and the expression ER target genes in grafted tumors. Figure S2 shows IGF1 rescues MED1KI/KI mammary gland development. Figure S3 shows inhibition of IGF1 signaling does not affect HER2+ mammary tumor cell apoptosis. Figure S4 shows inhibition of IGF1 reduces HER2+/ER+ human breast cancer cell migration, invasion and CSC formation. Figure S5 shows the relation between MED1 and HER2 status as well as clinical outcomes of breast cancer patients by analyzing public data.