Supplementary Figure S7 from TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation

Supplementary Figure S7 MiR-424 regulates many genes associated with EMT and cancer stemness. Effects of MCF12A cells stably expressing constitutive miR-424 on (A) known downregulated genes in an EMT signature (1) and on (B) known upregulated genes in a breast cancer stem cell signature (2), as demonstrated using RNA-seq with GSEA. TargetScan-predicted miR-424 binding sites (blue, asterisk) are statistically enriched in the downregulated genes, as assessed using a hypergeometric test with multiple correction permutations (A: p<10-5, B: p=0.002). Levels of TGFBR3, by qPCR, in (C) HMLE and (D) MCF12A cells stably expressing miR-424. SEM shown. **p<0.01, two-tailed unpaired t-test. (E) Flow cytometry for TGFBR3 after transfection of a rescue construct into SUM149PT cells expressing miR-424.