Supplemental Material from Cellular and Molecular Identity of Tumor-Associated Macrophages in Glioblastoma

Figure S1. Circulating Ly6CHi inflammatory monocytes diminish at terminal illness. Figure S2. Enhanced BM-derived cell infiltration in Cx3cr1GFP/GFP;Ccr2RFP/WT mice. Figure S3. RFP+ cells are only observed in the meninges in healthy naïve Cx3cr1GFP/WT;Ccr2RFP/WT mice. Figure S4. Bone marrow reconstitution efficiency as examined by flow cytometry. Figure S5. Significant up-regulated (red) and down-regulated (green) genes based on the pairwise comparisons. Figure S6. Loss of Ccl2 in stroma does not prolong survival of tumor-bearing mice. Figure S7. Immunohistochemistry staining and quantification of phosphor-histone 3 proliferating cells. Figure S8. Immunohistochemistry staining and quantification of Ki67+ proliferating cells. Figure S9. TUNEL staining of apoptotic cells. Figure S10. Survival curve of PDGF-driven tumor in WT, Ccl2+/- and Ccl2-/- mice. Supplementary Table S1. Combined Cx3cr1 and Ccr2 heterozygous knockout does not affect tumor development or survival. Supp. Table S2. Significantly enriched functional pathways in tumor-associated microglia. Supp. Table S3. Significantly enriched functional pathways in tumor-associated microglia and tumor-associate macrophages. Supp. Table S4. Significantly enriched functional pathways in tumor-associated macrophages.