Supplemental Figures 1-10, Supplemental Tables 1-6, Supplemental Materials and Methods. from ATDC/TRIM29 Drives Invasive Bladder Cancer Formation through miRNA-Mediated and Epigenetic Mechanisms

Supplemental Figure 1. ATDC mRNA expression in normal littermate (N) or CAG-ATDC transgenic bladders Supplemental Figure 2. Bladder urothelial lesions in CAG-ATDC transgenic line A of 8-12 months of age. Supplemental Figure 3. ATDC mRNA levels in CAG-ATDC tumors (T) are similar to human bladder cancers (UC9 and UC14) and ~20 fold higher than normal mouse (N) or human urothelial cell (TRTHU) expression. Mean{plus minus}SE, n = 3 for each column, * p < 0.05 compared to N. Supplemental Figure 4. ATDC immunostaining in two representative human invasive urothelial carcinomas demonstrates predominantly cytoplasmic staining (A) in most samples with rare tumors showing nuclear localization (B). Supplemental Figure 5. ATDC overexpression drives invasion in UC10 bladder cancer cell line. Supplemental Figure 6. PTEN immunostaining in three representative images of normal mouse bladder, CAG-ATDC mouse bladder or CAG-ATDC mouse tumor specimens demonstrating decreased PTEN expression in ATDC transgene expressing tissues Supplemental Figure 7. PTEN Quantitative methylation specific PCR. Supplemental Figure 8. Knockdown of ATDC results in reduction in DNMT3A, but not DNMT1 or DNMT3B in UC14 cell line as measured by immunoblot. Supplemental Figure 9. ATDC overexpression up-regulates DNMT3A, �-catenin and c-MYC mRNA expression and suppresses expression of PTEN. Supplemental Figure 10. Knockdown of MYC increases miR-29A and B1 in bladder cancer cell lines. Supplemental Table 1. Summary of bladder urothelial lesions in CAG-ATDC mouse line A. Supplemental Table 2. ATDC is significantly over-expressed in human bladder cancer. Supplemental Table 3. Genes up-regulated in all three CAG-ATDC invasive bladder tumors. Supplemental Table 4. Genes up-regulated in CAG-ATDC bladder cancers show significant overlap with the top 5-10% of overexpressed genes in bladder cancer in the Oncomine database. Supplemental Table 5. Molecular concepts showing enrichment with expression signature of genes over-expressed in CAG-ATDC bladder cancers through Oncomine analysis. Supplemental Table 6. ATDC and DNMT3A IHC Expression Correlation.