Data Supplement from Neuronal Pentraxin 2 Supports Clear Cell Renal Cell Carcinoma by Activating the AMPA-Selective Glutamate Receptor-4

Supplementary Figure 1. (A) QPCR for NPTX2 expression in an array of normal (n=4) and ccRCC (n=7) cell lines. All samples are normalized to a water negative control. Four shRNA lentiviral constructs (sh804, sh855, sh1316, sh1623) against NPTX2 were used to infect A498 cells. (B) Description of ccRCC cell line VHL mutational status. (C) QPCR for NPTX2 mRNA in each of the A498 NT vs. target lentiviral clones. (D) Western blot analysis for NPTX2 protein expression in all four A498 NPTX2 shRNA clones compared to NT control. Protein expression level quantitation is normalized to β-actin. (E) Proliferation (7 day) rescue assay of A498 cells transfected with either a human NPTX2 expression vector (+NPTX2) plasmid or empty vector (EV) control, and infected with NT control, sh1316 or sh1623 NPTX2 targeting lentivirus.