Supplementary Materials and Methods and Supplementary Figures 1 through 7 from Cystine Deprivation Triggers Programmed Necrosis in VHL-Deficient Renal Cell Carcinomas

Supplementary materials and methods. Figure S1: VHL renders renal cancer cell resistant to cystine deprivation-induced cell death. Figure S2: Cystine deprivation induces a programmed necrosis in VHL-deficient renal cancer cells. Figure S3: VHL represses TNFalpha expression to protect from Cystine-deprived necrosis. Figure S4: Metabolomic and transcriptional response to cystine deprivation. Figure S5: Activation of Src and p38 kinases is required for cystine-deprived necrosis. Figure S6: Mutual amplification between the Src-p38-Noxa and TNF-RIP1/3-MLKL pathways. Figure S7: Low doses of erastin share a similar mechanism as cystine deprivation to trigger programmed necrosis in ccRCC.