Supplemental Figures and Tables from Cancer-Associated Fibroblasts in Pancreatic Cancer Are Reprogrammed by Tumor-Induced Alterations in Genomic DNA Methylation

Table S1. Primer sequences Table S2. Promoter methylation status of 8 genes in 3 paired primary PDAC cells and CAFs measured by methylation specific PCR Table S3. SOCS1 methylation analyzed by pryosequencing in stroma dissected from PDAC tissues Table S4 Pyrosequencing results of five methylation sites on the SOCS1 promoter in MSCs or CAFs* before or after co-culture with human PDAC cells. Table S5. A list of clusters with a minimum of 5 genes that are both methylated and downregulated in CAFs upon interacting with PDAC tumor cells Table S6. Summary of genes with expression alterations (fold of change >1.5) in MSC cocultured with Panc10.05T vs. singly cultured MSC in both methylated genes (beta value > 0.5) and unmethylated genes (beta value < 0.5), respectively Figure S1. Representative pyrosequencing results. Figure S2. DNMT1 mRNA expression in MSC cells following shRNA knockdown of DNMT1. Figure S3. Pathways most significantly upregulated in CAFs upon co-culturing with PDAC tumor cells. Figure S4. SOCS1 expression in MSC cells following shRNA knockdown of SOCS1.