Figure S3 from Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7

Figure S3. Effect of P2X7R deletion on VEGF release. A, in vitro VEGF release from wt or P2X7R-silenced B16 cells. B, intra-tumor VEGF content of B16-wt or P2X7R-silenced tumors grown in wt or P2X7R-KO mice. VEGF was measured by ELISA. A, * p < 0.05 for B16 shRNA vs B16-wt. B, * p < 0.05 for P2X7R-wt + B16 shRNA vs P2X7R-wt + B16-wt; {section sign}{section sign} p < 0.01 for P2X7R-KO + B16 shRNA vs P2X7R-KO + B16-wt. C-F, VEGF staining of tumors from P2X7R-KO (C and D) or wt (E and F) mice. Specimens were fixed and stained with the anti-VEGF Ab as described in Materials and Methods.