Supplementary Video 1 from Overcoming Limitations in Nanoparticle Drug Delivery: Triggered, Intravascular Release to Improve Drug Penetration into Tumors

AVI file - 41.7MB, Real-time drug delivery of free doxorubicin and Dox-TSL in heated FaDu tumor tissue. The blood vessels are highlighted in green and drug is red. The video shows drug delivered over the first 20min, at 20x magnification. Free drug quickly disperses throughout the tissue and then is reabsorbed into the vasculature, leaving a low drug concentration behind in the tumor cells. In the Dox-TSL treated tissue, drug is delivered uniformly across the tumor tissue, with visible uptake in tumor and endothelial cell nuclei. Drug in the Dox-TSL treated mouse continues to accumulate in the tumor tissue over the 20min period. (Download before playing in IE8)