Supplementary Fig. 1 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

PARP1 IHC staining. (a) PARP1 staining of frontal cortex autopsy tissue of a diffuse midline glioma patient. Only a subset of cells show PARP1 staining, while others are negative. Neurons show staining of the nucleoli, while cells of oligodendritic/astrocyte nature show PARP1 expression in the entire nucleus. Other cells, inclusing endothelial cells, show no PARP1 expression. The corresponding H&E is from the same area as the PARP1 stained image. (b) Physiological, non-tumor related PARP1 expression was observed in the brain of ntv-a;p53fl/fl mice in the Purkinje cell layer in the cerebellum and the dentate gyrus. Other brain areas showed low levels of PARP1 expression. (c) Example of PARP1 and H&E staining of tumor bearing ntv-a;p53fl/fl mice injected with DF1 cells at 4-6 weeks of age.