Supplemental Figures 1-9 and Tables 1 and 2 from FOXP3 Controls an miR-146/NF-κB Negative Feedback Loop That Inhibits Apoptosis in Breast Cancer Cells

Supplemental Figures 1-9 and Tables 1 and 2. Supplemental Table 1: Identification of potential FOXP3-targeted microRNAs in breast cancer cells. Supplemental Table 2: Primer sequences used in this study. Supplemental Figure 1: Expression profiles of IRAK1 and TRAF6 in MCF10A cells with miR-146a/b inhibitors. Supplemental Figure 2: Expression of nuclear FOXP3 in FOXP3-Tet-off MCF7 cells. Supplemental Figure 3: The expression profile of NF-κB target genes after FOXP3 induction in FOXP3-Tet-off MCF7 cells. Supplemental Figure 4: Identification of potential FOXP3-binding sites in promoter regions of miR-146a/b in the FOXP3-Tet-off MCF7 cells by whole-genome ChIP-seq. Supplemental Figure 5: Quantitative analysis of FOXP3-binding sites in the potential promoter region of human miR-146b in FOXP3-Tet-off MCF7 cells. Supplemental Figure 6: Specific binding of FOXP3 to the second forkhead-binding motif (GCAAATA) in the miR-146a promoter. Supplemental Figure 7: MiR-146a/b upregulation by FOXP3 transfection in MDA-MB231 cells. Supplemental Figure 8: The mRNA expression levels of IRAK1and TRAF6 in metastatic breast tumor cells after administration of miR-146a/b inhibitors. Supplemental Figure 9: A potential mechanism by which FOXP3 regulates the NF-κB-miR-146a/b feedback loop through IRAK1 and TRAF6 in tumor growth or regulates CXCR4/MMP9 in tumor metastasis.