Supplementary Figures S1-S10 from Endothelial Thermotolerance Impairs Nanoparticle Transport in Tumors

Tumor accumulation of AS750 after PEG-NR heat exposure with varying delta T (S1); Diminished window of tumor transport after re-exposure to PEG-NR heating (S2); Animal weights during PEG-NR and doxorubicin liposome therapeutic trial (S3); Transvascular transport in tumors after single exposure and re-exposure to PEGNR heating (S4); Vascular endothelial growth factor (VEGF) expression in PEG-NR heated tumors (S5); Endothelial cell F-Actin dynamics during heat exposure and recovery (S6); Visualizing PEG-NRs in the tumor microenvironment using multiphoton microscopy (S7); Cargo accumulation in tumors with Hsf1+/+ and Hsf1-/- vasculature after single exposure to PEG-NR heating (S8); HSF1 expression in tumor-associated vasculature following PEG-NR heating (S9); Size-dependent macromolecular extravasation from the unheated vasculature (S10).