Supplementary Figures 1 - 5 from Curcumin Promotes Autophagic Survival of a Subset of Colon Cancer Stem Cells, Which Are Ablated by DCLK1-siRNA

PDF file - 2102KB, Colon cancer cells positive for DCLK1, co-express CD44, while Lgr5+ve cells do not (S1). Localization of cells expressing DCLK1/Lgr5/CD44 within spheroidal growths (S2). Effect of curcumin on relative expression of indicated proteins in HCT-116 cells, growing as either 2D monolayers, or 3D spheroids or xenografts in athymic nude mice (S3). Curcumin induces autophagy in HCT-116 cells in culture (S4). Effect of curcumin�DCLK1-siRNA on levels of LC3-I/II and PCNA staining and on stem cell populations in HCT-116 xenografts (S5).