Supplementary Figure 4 from Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2

PDF file - 67K, A375 cells were treated with L-NIL (300 μM and 1000 μM) or the NO scavenger PTIO (300 μM) for 24 hours prior to harvest and preparation of protein lysates. Western Blot analyses of AKT phosphorylation at serine 473 and total AKT demonstrated lack of downregulation of AKT phosphorylation under conditions which strongly reduce downstream mTOR pathway activation.