Supplementary Figures 1 - 7 from Neuropilin-2 Is Upregulated in Lung Cancer Cells during TGF-β1–Induced Epithelial–Mesenchymal Transition

PDF file - 1864K, NRP2 is stably up-regulated by TGFβ in lung cancer cells without increasing the halflife of mRNA or protein (S1); NRP2 up-regulation is blocked by inhibition of TβRI, AKT, ERK and ZEB1, but not by expression of inhibitory SMAD7 (S2); NRP2 knockdown blunts TGFβ-mediated transcriptional responses, ERK and AKT phosphorylation but does not influence SMAD activation (S3); NRP2 knockdown blocks cell scattering induced by TGFβ (S4); Immunohistochemical detection of NRP2 and cytokeratin in TGFβ exposed xenograft tumors with NRP2 or control knockdowns (S5); E-cadherin and NRP2 immunostaining of samples from a tumor microarray (S6); Predicted miRNA target sites in the 3' UTR of NRP2 and up-regulated NRP2 protein levels in hnRNP E1 knockdown cells (S7).