Supplementary Figures S1-S12 and Legends from Notch Suppresses Angiogenesis and Progression of Hepatic Metastases

Supplementary Figures S1-S12 and Legends. Supplementary Figure S1. Combined Inhibition of Notch and VEGF disrupts interaction of pericytes with endothelial cells in primary tumors Supplementary Figure S2. Inhibition of Notch increases liver metastases. Supplementary Figure S3. Inhibition of Notch increases liver metastases as measured by bioluminescence in liver homogenates. Supplementary Figure S4. Notch blockade does not increase metastases to other organs. Supplementary Figure S5. N1D does not affect proliferation, invasion or migration of neuroblastoma cell lines. Supplementary Figure S6. N1KD decreases Hes1 and Hey1 but does not affect proliferation of NGP cells Supplementary Figure S7. Increased vasculature of liver metastases with Notch blockade Supplementary Figure S8. Activation of HSCs in liver metastases with Notch blockade Supplementary Figure S9. SEC expression of ICAM-1 is regulated by Notch Supplementary Figure S10. AdN1D increases expression of liver ICAM-1 but does not increase retention of tumor cells in the liver. Supplementary Figure S11. Notch1 decoy variants increase proliferation of mSEC but not NGP cells Supplementary Figure S12. Jag1 siRNA decreases expression of Jag1 in NGP cells.