Supplementary Table 1, Figures 1 - 9 from Human Pancreatic Cancer Tumors Are Nutrient Poor and Tumor Cells Actively Scavenge Extracellular Protein

Table S1. Amino acid concentrations in pancreatic tumor and benign adjacent tissues Figure S1. Quantification of macropinocytosis in human pancreatic tumors. Figure S2. Few macropinosomes are present in the normal pancreatic tissue adjacent to PDAC tumors. Figure S3. Ras-mutant pancreatic cells are capable of indefinite growth in medium lacking leucine supplemented with physiological levels of serum protein. Figure S4. Ras-mutant pancreatic cells are capable of indefinite growth in medium lacking lysine or phenylalanine supplemented with physiological levels of serum protein. Figure S5. Growth of KRPC cells in leucine-free medium with 50 g/L serum protein cannot be explained by the presence of contaminating leucine. Figure S6. Stable isotope tracing reveals significant contribution of serum protein to amino acid pools in human PDAC cells. Figure S7. EIPA, an inhibitor of macropinocytosis, reduces intracellular (A) and extracellular (B) serum protein-derived amino acid pools in a dose-dependent fashion. Figure S8. Fractional contribution of serum protein-derived nitrogen to intracellular amino acid pools. Figure S9. Amino acid frequency in bovine serum albumin relative to human proteome.