Supplementary Figures S1-S13 and Table S1 from KIAA1324 Suppresses Gastric Cancer Progression by Inhibiting the Oncoprotein GRP78

Supplementary Figures S1-S13 and Table S1. Confirmation of KIAA1324 mRNA expression in primary tissues from gastric cancer (GC) patients and GC cell lines (S1). Analysis of correlation between copy number variation (CNV) and KIAA1324 mRNA expression in gastric cancer cell lines (S2). KIAA1324 enhanced cisplatin- and etoposide-induced apoptosis of gastric cancer cells (S3). KIAA1324 knockdown reduced staurosporine-induced apoptosis (S4). KIAA1324 did not have a considerable effect on cell cycle (S5). KIAA1324 did not affect autophagy of gastric cancer cells (S6). KIAA1324 â–³C caused growth inhibition and apoptosis in gastric cancer cells (S7). KIAA1324 â–³N did not induce apoptosis of gastric cancer cells (S8). 1-303 a.a. of KIAA1324 was required for KIAA1324-mediated apoptosis (S9). Subcellular localization of KIAA1324 (S10). 1-303 a.a. of KIAA1324 bound to GRP78. (A) A schematic model of KIAA1324 fragments (S11). GRP78 knockdown induced apoptosis of MKN28 cells (S12). A schematic model of the anti-tumorigenic ability of KIAA1324 through regulation of GRP78 activity (S13). Semi-quantitative and quantitative RT-PCR primers (Table S1).