A metabolomic view on local climate adaptation: Latitudinal divergence of heat and drought responses in a coastal plant
biorxiv(2022)
摘要
Studying natural variation in multi-stress resistance is central for predicting and managing the population dynamics of wild plant species under rapid global change. Yet, it remains a challenging goal in this field to integrate knowledge on the complex biochemical underpinnings for the targeted ‘non-model’ species. Here, we studied latitudinal divergence in combined drought and heat stress resistance in European populations of the dune plant Cakile maritima , by combining comprehensive plant phenotyping with metabolic profiling via FT-ICR-MS and UPLC-TQ-MS/MS.
We observed pronounced constitutive divergence in growth phenology, leaf functional traits and defence chemistry (glucosinolates, alkaloids) among population origins. Most importantly, the magnitude of growth reduction under stress was partly weaker in southern plants and associated with divergence in plastic growth responses (root expansion, leaf abscission) and the stress-induced modulation of primary and specialized metabolites with known central functions not only in plant abiotic but also biotic stress resistance.
Our study supports that divergent selection has shaped the constitutive and stress-induced expression of numerous morphological and biochemical functional traits to mediate higher abiotic stress resistance in southern Cakile populations, and highlights that metabolomics is a powerful tool to explore the mechanistic underpinnings of local stress adaptation in ‘non-model’ species.
Highlight Plant defence chemistry and its modulation by abiotic stress exhibits latitudinal clines across natural populations of a coastal plant.
### Competing Interest Statement
The authors have declared no competing interest.
* D
: Water treatment level control
D1
: Water treatment level drought
FDR
: False discovery rate
FT-ICR-MS
: Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry
(G)LMM
: (Generalized) linear mixed effects model
H
: Temperature treatment level control
H1
: Temperature treatment level heat
LDMC
: Leaf dry matter content
ON
: Plant origin level north
OS
: Plant origin level south
RH
: Relative air humidity
RSR
: Root shoot ratio
SLA
: Specific leaf area
UPLC-TQ-MS/MS
: Ultra Performance Liquid Chromatography-Triple Quadrupole-Mass Spectrometry
VPD
: Vapour pressure deficit
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关键词
local climate adaptation,drought responses,metabolomic view
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