Genetic polymorphisms of TNF-alpha, IL-6, and IL-10 in female elderly patients with chronic temporomandibular disorder pain

Aim: To verify the association of the TNF-alpha, IL-6, and IL-10 polymorphisms with chronic temporomandibular disorder pain development in female elderly patients. Methods: Participants were evaluated according to Diagnostic Criteria for Temporomandibular Disorders. The genomic DNA was extracted from blood according to the Salting Out method followed by a quantification using the NanoDrop spectrophotometer. The -308G/A TNF-alpha polymorphism analysis was performed by the polymerase chain reaction-restriction fragment length polymorphism technique, the determination of -174G/C IL-6 polymorphism was performed by polymerase chain reaction, and the evaluation of the -1082A/G IL-10 polymorphism was carried out by polymerase chain reaction-allele-specific amplification. Data were analyzed using the BioEstat 5.3 software. Results: The -308G/A TNF-alpha polymorphism showed a significant difference when genotypes of cases with chronic temporomandibular disorder pain and controls were compared (p = .0256). There was a lack of association regarding the -174G/C IL-6 polymorphism (p = .2865) however, a positive association between the -1082A/G IL-10 polymorphism with chronic temporomandibular disorder was observed (p = .0200). Conclusion: The analyzed data of elderly subjects support the possible involvement of the GA genotype of the -308G/A TNF-alpha and the AA genotype of the -1082A/G IL-10 polymorphisms in the pathogenesis of chronic temporomandibular disorder pain.