Nematode and Strepsipteran Parasitism in Bait-Trapped and Hand-Collected Hornets (Hymenoptera, Vespidae, Vespa ).

The parasitism of two groups of host-manipulating parasites of hornets was examined in Kyoto, Japan. (661 individuals), (303), (457), (158), (57), and (4) were collected either by bait trap or hand collection with an insect net, and examined for their parasites. An endoparasitic nematode, was isolated from three overwintered gynes of and a gyne of . While endoparasitic insects, spp., were recovered from 13 , 77 , two and three , and those recovered from and others were molecularly identified as and , respectively. Comparing parasitism level and capturing methods, the parasitism level was significantly higher in trapped hosts than in hand-collected ones, suggesting that stylopized hosts are more strongly attracted to the food source (bait trap) compared with unparasitized hosts. The genotypes of were identical to each other, and near identical to its type population. While each of the two spp. showed four mitochondrial DNA haplotypes. A phylogenetic comparison suggested that haplotypes found in the present study are close to those previously reported from Japan and other Asian countries.