Expression and purification of MERS-CoV envelope protein, an essential viroporin, using the baculovirus expression system.

Entedar Alsaadi,Dhafer Alghezi,Ian Jones

Iranian journal of microbiology（2023）

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摘要

Purified full length recombinant MERS-CoV E protein can be isolated by IMAC and is suitable for further functional, biophysical or immunological studies.

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关键词

Baculovirus,Coronaviruses,Envelope protein,Immobilised metal-affinity chromatography,Insect cells,MERS-CoV,Middle east respiratory syndrome

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