Actinophytocola gossypii sp. nov. and Streptomyces gossypii sp. nov., two novel actinomycetes isolated from rhizosphere soil of cotton.

Two Gram-positive, aerobic and non-motile actinomycetes, designated S1-96 and N2-109, were isolated from soils collected from a cotton field. They are described as representing two novel species of genera and through a polyphasic approach. Analysis of 16S rRNA gene sequences revealed that strains S1-96 and N2-109 showed highest similarity to CGMCC 4.4663 (99.10 %) and BNT558 (98.21 %), respectively. Phylogenetic analyses based on 16S rRNA and core genes confirmed the close relationships of these strains. Genomic analyses further supported the novel taxonomic delimitation of these two species based on digital DNA-DNA hybridization and average nucleotide identity. Strains S1-96 and N2-109 contained MK-9(H) and MK-9(H) as the most abundant menaquinone, respectively. High abundances of iso-fatty acids were detected in both strains, which was similar to their close relatives. Physiological and polar lipid analyses also revealed differences between these strains and their phylogenetic neighbours, supporting their taxonomic delimitation as novel species. The names sp. nov. (type strain S1-96=JCM 34412=CGMCC 4.7707) and sp. nov. (type strain N2-109=JCM 34628=CGMCC 4.7717) are proposed.