Real-time calcium uptake monitoring of a single renal cancer cell based on an all-solid-state potentiometric microsensor.
Frontiers in bioengineering and biotechnology(2023)
摘要
In addition to many cellular processes, Ca is also involved in tumor initiation, progression, angiogenesis, and metastasis. The development of new tools for single-cell Ca measurement could open a new avenue for cancer therapy. The all-solid-state calcium ion-selective microelectrode (Ca-ISμE) based on carbon fiber modified with PEDOT (PSS) as solid-contact was developed in this work, and the characteristics of the Ca-ISμE have also been investigated. The Ca-ISμE exhibits a stable Nernstian response in CaCl solutions in the active range of 1.0 × 10 - 3.1 × 10 M with a low detection limit of 8.9 × 10 M. The Ca-ISμE can be connected to a patch clamp to fabricate a single-cell analysis platform for calcium monitoring of a single renal carcinoma cell. The calcium signal decreased significantly (8.6 ± 3.2 mV, n = 3) with severe fluctuations of 5.9 ± 1.8 mV when the concentration of K in the tumor microenvironment is up to 20 mM. The results indicate a severe cell response of a single renal carcinoma cell under high K stimuli. The detection system could also be used for single-cell analysis of other ions by changing different ion-selective membranes with high temporal resolution.
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关键词
calcium,ion-selective microelectrodes,renal cell carcinomas,single-cell analysis,tumor microenvironment
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