Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer.

Maria do Carmo Greier,Annette Runge,Jozsef Dudas,Lukas Carpentari,Volker Hans Schartinger,Avneet Randhawa,Melissa Mayr,Monika Petersson,Herbert Riechelmann

Frontiers in oncology（2023）

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摘要

HNC SC can be preserved for up to 7 days using the tested cultivation media. Cell viability was best preserved with addition of PRF. HNC SC are a versatile experimental tool to study physiology and drug actions. Autologous PRF can help simulate realistic conditions .

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关键词

culture media,cultured neoplastic cells,head and neck cancer,platelet rich fibrin,tumor microenvironment

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