The Use of Microsatellites in the Management of Captive Gibbons

Genetic profiling can validate pedigrees and reveal genetic diversity/inbreeding within populations. We have developed 12 autosomal microsatellite markers that can be used to DNA profile gibbon species. The panel generated full profiles for 39 individuals currently or previously housed at Twycross Zoo, UK, representing five species across three genera. The study is extending to a further approximate 100 samples, including three additional species, from captive populations across Europe. The panel’s cross-species utility allows for a single protocol to be used for all DNA profiling, avoiding the need for species-specific testing. In addition, the panel resolved an issue of uncertain paternity in a breeding group, with direct implications for group management and welfare. The loci reported here yielded profiles from blood, tissue and non-invasive hair samples. Positive impact on the viability and sustainability of captive breeding programmes is anticipated, by clarifying cryptic relatedness and informing future pairings. Potential exists for field application in investigating population dynamics, mating behaviours, relatedness and dispersal patterns, as well as assessing the impact of anthropogenic disturbances on the genetic architecture of populations. This established panel, effective across multiple gibbon species and genera, presents an affordable and expedient tool for research and captive management.