Development of a rapid reverse genetics system for feline coronavirus based on TAR cloning in yeast.
Frontiers in microbiology(2023)
摘要
Using this system, the viruses could be rescued rapidly and stably without multiple cloning steps. Considering its speed and ease of manipulation in virus genome assembly, the reverse genetics system developed in this study will facilitate the research of the feline coronaviruses pathogenetic mechanism and the vaccine development.
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关键词
FCoV,FIPV,reverse genetics,transformation-associated recombination,yeast
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