Size exclusion chromatography to evaluate in vitro proteolysis: A case study on the impact of microstructure in pulse powders

•SEC delivers in-depth insight into proteolysis patterns complementary to OPA.•Bioaccesible protein release reached a plateau during the small intestinal phase.•Proteolysis in the insoluble (inaccessible) fraction proceeded throughout digestion.•Pulse microstructure dictated protein hydrolysis and solubilization kinetics.•Mw evolution in insoluble fraction depended on pulse type and microstructure.