Direct Thin Layer Chromatographic Enantioseparation of Active Pharmaceutical Ingredients via Non-Covalent Diastereomers

Worldwide thin layer chromatography (TLC) is still in very much use in various different application fields (e.g. natural products chemistry, monitoring the organic/enantioselective synthesis, and routine analysis in analytical laboratories). Herein is presented an account of the efficient resolution/enantioseparation of racemic and non-racemic mixtures of several different types of active pharmaceutical ingredients, and biologically important compounds by TLC using a direct approach along with quantitative determination and isolation of native forms via non-covalent diastereomer formation. Different approaches and mechanisms of direct enantioseparation using different chiral reagents are discussed. It provides a route for a wide range of chiral stationary phases (CSPs) which can be in-home thought out, produced, assessed, and customized. The achievements and new challenges in the field are presented. Besides, it presents a very useful coverage for the non-specialists to the subject who want to understand where this study fits into the existing literature in this general area.