Purification of Antibodies Using Novel Convecdiff Membranes Part 1: Providing High Binding Capacity at Short Residence Time and Low Pressure Drops

Convecdiff membranes, a new type of chromatographic material that combines high protein-binding capacity with high productivity while providing scalability, are introduced. The convecdiff membrane prototypes presented in this study reach a dynamic binding capacity of approximately 40 g/L at residence times in the seconds range while pressure drop is below 2.5 bar at a bed height of several millimeters. The robustness of the new material is proven up to 200 cycles. The material performance regarding yield, impurity reduction, and other results observed during cycling is comparable to the performance of established materials, and no performance decline occurred during cycling. With all these characteristics, the material is well suited for protein bind-and-elute applications ranging from laboratory to production scale.