Development of more potent anti-microbial drugs from extracts of five medicinal plants resistant to S. aureus in human fluids: an ex vivo and in vivo analysis

Methicillin-resistant staphylococcal infections are a serious public health concern worldwide, especially in our region, due to the unavailability of accurate therapeutics. The aim of this study was to determine the resistance pattern of Staphylococcus aureus isolated from clinical and asymptomatic specimens and to identify antimicrobial agents against methicillin resistant S. aureus strains. 47 samples (pus, urine, blood, nose and milk) together from diverse healthcare hospitals in Faisalabad for location of S. strains . Aureus by biochemical examination. The results of these biochemical tests showed that 21 of 47 samples were positive for S. aureus . A resistance study found that 10 of 21 S. aureus isolates were resistant to oxacillin. Twenty-five medicinal plants were collected from different parts of Saudi Arabia, air-dried and extracted with methanol. These plant extracts were then screened for potential against oxacillin-resistant S. aureus . In addition, the semi-maximum inhibitory concentration (IC50) of effective medicinal plant extracts was measured using the micro-dilution method . Methanol extracts from five plants ( Centratherum antherminticum , Eucalyptus globulus , Lawsonia inermis , Punica granatum and Rubia cordifolia ) have been shown to have anti-bacterial activity against clinically isolated S. oxacillin resistant strains. It was done. The IC50 for these methanol extracts of S. aureus ranged from 0.250 to 4.30 mg/mL. Our results suggest that various medicinal plants contain potential anti-bacterial agents against S. aureus , which may contribute to the development of more effective agents against multidrug resistant S. aureus infections. It might help.