Isolation of Volatiles in Honeysuckle by Multiple Approaches with Determination by Gas Chromatography-Mass Spectrometry (GC-MS) and Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS-SPME/GC-MS)

The volatile components of honeysuckle were determined using hydro-distillation (HD), water extraction coupling rectification (WER), and water extraction coupling rectification with solvent extraction (WER-SE). Gas chromatography-mass spectrometry (GC-MS) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS) identified 127 compounds. There were 27 common compounds extracted by these methods. A heatmap of the 27 common compounds indicates that WER-SE and WER were complementary approaches. In addition, 59 compounds were detected for the first time in honeysuckle. The results of this study provide a reference for the determination of volatile components in plants.