Supplementary Figure 2 from DNA Damage-Inducible Gene, Reprimo Functions as a Tumor Suppressor and Is Suppressed by Promoter Methylation in Gastric Cancer

PDF file - 216K, Supplementary Figure. 2. Invasion assay and cell cycle assay. (A) Invasion assay. With AZ-m cells as 1.0, the relative invasive rates were shown. Bars, 200 �m; *, P<0.05; Error bars; SD; NS, not significant. (B) Cells without Reprimo methylation (AZ521 or H111 cells) were transfected with pcDNATM3.1-mock or pcDNATM3.1-Reprimo, and the stable cells were established by G418 selection. For cell cycle assay, after cells were synchronized for 48 hours in serum free medium, the cells were then cultured in complete medium for 24 hours. The percentage and SD of cell cycle phases are shown in each panel. *, P<0.05; Error bars; SD.