Supplementary Figures 1 through 15 and Supplementary Table 1 from Drug Repurposing Identifies a Synergistic Combination Therapy with Imatinib Mesylate for Gastrointestinal Stromal Tumor

Figure S1. Analysis of combination index (CI) value of IM combined with FDA-approved drugs in GIST-T1 cells. Figure S2. Analysis of CI value of IM combined with FDA-approved drugs in GIST 882 cells. Figure S3. Analysis of CI value of IM combined with FDA-approved drugs in GIST T1-10R cells. Figure S4. Drug combination assays using the checkerboard experimental design. Figure S5. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with idarubicin on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S6. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with digoxin on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S7. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with bortezomib on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S8. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with auranofin on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S9. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with ouabain on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S10. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with gentian violet on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S11. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with carbimazole on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S12. Representative dose-effect, median-effect and normalized isobologram (Chou-Chou Plot) plots for IM in combination with F-AMP on A) GIST-T1, B) GIST 882, and C) GIST T1-10R cells. Figure S13. Apoptosis analysis of GIST cells treated with vehicle, F-AMP, IM, or the drug combination. Figure S14. Mice body weights of each treatment group in the in vivo study. Figure S15. Representative H and E staining photographs of livers, kidneys and spleens from each treatment group in the in vivo study. Table S1. Optimized drug doses for the apoptosis assays.