Supplementary Information from Discovery of a Novel Small-Molecule Inhibitor that Targets PP2A–β-Catenin Signaling and Restricts Tumor Growth and Metastasis

SUPPLEMENTARY FIGURE S1: General information and rationale of synthesis of hybrid molecules of Chalcone and Semicarbazone SUPPLEMENTARY FIGURE S2: Detailed qualitative analysis of compounds of Chalcone and Semicarbazone hybrid series Supplementary Figure S2a: NMR spectra and structure of compounds Supplementary Figure S2b: HPLC chromatogram of compounds Supplementary Figure S2c: Crystal structure of representative CS-24 SUPPLEMENTARY FIGURE S3: Dose dependent cytotoxic potential of CS-11 and parent compound 8e on different breast cell lines SUPPLEMENTARY FIGURE S4: Densitometric quantification and statistical analysis of western blots shown in Figure 2d SUPPLEMENTARY FIGURE S5: Effect of CS-11 on colon cancer cell growth inhibition and breast cancer cell migration Supplementary Figure S5a: IC50 and Dose dependent cytotoxic potential of CS-11 and parent compound 8e on SW620 colon cancer cell line Supplementary Figure S5b: Effect of CS-11 on breast cancer cell migration in in vitro scratch assay SUPPLEMENTARY FIGURE S6: Densitometric quantification and statistical analysis of western blots shown in Figure 5a (S6a) and 5c (S6b) SUPPLEMENTARY FIGURE S7: Densitometric quantification and statistical analysis of western blots shown in Figure 6a SUPPLEMENTARY TABLE S1: Crystal data and structure refinement details for representative compound CS-24 SUPPLEMENTARY TABLE S2: Cytotoxic potential of CS series on different cancer cell lines Supplementary Table S2a: Differential response of Chalcone Semicarbazone hybrids series on different cancer cells Supplementary Table S2b CS-11 induces breast cancer cell selective cytotoxic effects SUPPLEMENTARY METHODS Supplementary Method S1: Detailed procedure of compound synthesis Supplementary Method S2: X-Ray data collection and structure refinement details Supplementary Method S3: Methodology for pharmacokinetic studies